A simple, precise, accurate and reproducible spectrophotometric method has been developed for simultaneous estimation of Cefixime (CEF) and Levofloxacin (LEVO) by employing first order derivative zero crossing method in Methanol. The first order derivative absorption at 290 nm (zero cross point of Cefixime) was used for quantification of Levofloxacin and 265 nm (zero cross point of Levofloxacin) for quantification of Cefixime. The linearity was established over the concentration range of 4-20 µg/ml and 2.5-10.5 µg/ml for Cefixime and Levofloxacin with correlation coefficient r2 0.9984 and 0.9996, respectively. The mean % recoveries were found to be in the range of 98.60% –101.19% and 98.04%–101.66% for Cefixime and Levofloxacin, respectively. The proposed method has been validated as per ICH guideline and successfully applied to the estimation of Cefixime and Levofloxacin in bulk and in market formulation.
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